Title | CCR1/CCL5 (RANTES) receptor-ligand interactions modulate allogeneic T-cell responses and graft-versus-host disease following stem-cell transplantation. |
Publication Type | Journal Article |
Year of Publication | 2007 |
Authors | Choi, SW, Hildebrandt, GC, Olkiewicz, KM, Hanauer, DA, Chaudhary, MN, Silva, IA, Rogers, CE, Deurloo, DT, Fisher, JM, Liu, C, Adams, D, Chensue, SW, Cooke, KR |
Journal | Blood |
Volume | 110 |
Issue | 9 |
Pagination | 3447-55 |
Date Published | 2007 Nov 1 |
ISSN | 0006-4971 |
Keywords | Animals, Chemokine CCL5, Gene Expression Regulation, Graft vs Host Disease, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, Protein Binding, Receptors, CCR1, Stem Cell Transplantation, Survival Analysis, T-Lymphocytes, Transplantation, Homologous |
Abstract | Acute graft-versus-host disease (GVHD) and leukemic relapse are serious complications of allogeneic stem-cell transplantation (SCT). Recruitment of activated T cells to host target tissues or sites of leukemic infiltration (graft-versus-leukemia [GVL]) is likely mediated by chemokine receptor-ligand interactions. We examined the contribution of donor cell CCR1 expression to the development of GVHD and GVL using a well-established murine SCT model (B6 --> B6D2F1) and CCR1-deficient mice (CCR1(-/-)). Allo-SCT with CCR1(-/-) donor cells significantly reduced systemic and target organ GVHD severity, and CCR1 expression on both T cells and accessory cells contributed to GVHD mortality. Significant GVL activity was preserved following CCR1(-/-) SCT, but the survival advantage diminished with increasing tumor burden. We then explored the effects of CCR1 expression on allo-specific T-cell responses. Although cytolytic effector function was maintained on a per-cell basis, T-cell proliferation and IFNgamma secretion were significantly reduced both in vivo and in vitro. T-cell function was partially dependent on interactions between CCR1 and CCL5. Collectively, these data demonstrate that CCR1 expression on donor cells contributes to the development of both GVHD and GVL, and suggest that CCR1/CCL5 receptor-ligand interactions modulate allo-specific T-cell responses occurring in this context. |
DOI | 10.1182/blood-2007-05-087403 |
Alternate Journal | Blood |
PubMed ID | 17641205 |
PubMed Central ID | PMC2200916 |
Grant List | 5R01 HL 072258-04 / HL / NHLBI NIH HHS / United States |
Research reported in this publication was supported by the National Cancer Institutes of
Health under Award Number P30CA046592. The content is solely the responsibility
of the authors and does not necessarily represent the official views of the
National Institutes of Health.
Research reported in this publication was supported by the National Cancer Institutes of
Health under Award Number P30CA046592 by the use of the following Cancer Center
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